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International Desk: Zagro Asia Limited has recently conducted testing of its key disinfectant, Ultraxide™, against African Swine Fever (ASF).
Zagro believes that at this point in time, only a sound biosecurity measure is feasible against ASF, with no vaccine nor other medical treatment currently available.
ASF has been causing a large number of infections in pigs across Europe, China and Southeast Asia with devastating effect to the farms and to the Economy.
Zagro Asia Limited had commissioned for independent evaluation of Ultraxide™ against the ASF virus in Spain, in accordance with the EN 14675 test method, which was modified to enable testing against the ASF virus.
The testing was done in a European Union reference laboratory for ASF.
Based on the findings, Ultraxide™ had achieved complete inactivation of the ASF virus at a 1:300, 1:200, and at 1:100 dilution rate at the exposure time of 5 minutes.
The test method employed included the presence of organic matter and hard water in order to assess Ultraxide™’s performance and suitability for use in various farm set ups across the globe.
Table 1. Titer reduction of ASFV after incubation with Ultraxide™ (1:300, 1:200and 1:100 dilutions) in a suspension test at 10ºC of temperature (contact time:5 or 10 minutes), in the presence of the interference substance SFB 3% or none.
| Product | Concentration | Contact Time Min | Soiling | Level of cytotoxicity | Lg TCID 50 after contact time | Log 10 reduction after contact time |
| ASFV positive control |
– |
5 min |
3 % FBS |
0 |
9.0
|
– |
| ASFV positive control |
– |
10 min |
3 % FBS |
0 |
8.0 |
– |
| ASFV positive control |
– |
5 min |
– |
0 |
8.9 |
– |
| ASFV positive control |
– |
10 min |
– |
0 |
8.0 |
– |
| Zagro Ultraxide™ | 1:100 | 5 min | 3 % FBS | 0 | 3.5 | 5.5 |
| Zagro Ultraxide™ | 1:200 | 5 min | 3 % FBS | 0 | 3.5 | 5.5 |
| Zagro Ultraxide™ | 1:300 | 5 min | 3 % FBS | 0 | 3.5 | 5.5 |
| Zagro Ultraxide™ | 1:100 | 10 min | 3 % FBS | 0 | 3.5 | 4.5 |
| Zagro Ultraxide™ | 1:200 | 10 min | 3 % FBS | 0 | 3.5 | 4.5 |
| Zagro Ultraxide™ | 1:300 | 10 min | 3 % FBS | 0 | 3.5 | 4.5 |
| Zagro Ultraxide™ | 1:100 | 5 min | – | 0 | 3.5 | 5.4 |
| Zagro Ultraxide™ | 1:200 | 5 min | – | 0 | 3.5 | 5.4 |
| Zagro Ultraxide™ | 1:300 | 5 min | – | 0 | 3.5 | 5.4 |
| Zagro Ultraxide™ | 1:100 | 10 min | – | 0 | 3.5 | 4.5 |
| Zagro Ultraxide™ | 1:200 | 10 min | – | 0 | 3.5 | 4.5 |
| Zagro Ultraxide™ | 1:300 | 10 min | – | 0 | 3.5 | 4.5 |
Based on the test results with reference to European standard EN14675, we can conclude that:
The disinfectant Ultraxide™ possesses virucidal activity for the referenced strain of African swine fever virus (Ba71V) at 1:300 (one part of test product plus 300 parts of diluents), 1:200 (one part of test product plus 300 parts of diluents) and 1:100 dilution (one part of test product plus 100 parts of diluents).
The result shows that it reduces >4 log10 of ASFV cytopathic effect, as required by the standard EN14675.
The results provided confirmation of the efficacy of Ultraxide™ in deactivating the African Swine Fever virus rapidly, not only in a laboratory set up but also in real life farm settings.
Zagro Asia Limited is a leader in agrisolutions with footprints in more than 70 countries.
Extensively used in farming communities worldwide, the company’s range of disinfectants, specifically Ultraxide™, are tested to destroy animal viruses.
As part of Zagro’s commitment to providing biosecurity solution and support for ASF control with Ultraxide™, Zagro’s team of veterinarians can be contacted via email (support@zagro.com) or via Whatsapp (+65 9653 1942).
Visit our website at www.zagro
